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| Registros recuperados : 27 | |
| 3. |  | FRATA, M. M.; FERRONATO, G. A.; RINCÓN, J. A. A.; LOPES, I. A. R.; PRADIEÉ, J.; PEGORARO, L. M. C. Adição de lipoproteína de alta densidade na maturação in vitro de oócitos bovinos. In: CONGRESSO DE INICIAÇÃO CIENTÍFICA, 26.; ENCONTRO DE PÓS-GRADUAÇÃO UFPEL, 19.; SEMANA INTEGRADA DE ENSINO, PESQUISA E EXTENSÃO, 3., 2017, Pelotas. Anais... Pelotas: UFPel, 2017.| Biblioteca(s): Embrapa Clima Temperado. |
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| 4. | | FERRONATO, G. A.; RINCÓN, J. A. A.; BORGES, M. A.; SCHNEIDER, A.; PEGORARO, L. M. C.; CORRÊA, M. N. Efeito da lipoproteína de alta densidade durante a maturação oocitária sobre o status oxidativo de oócitos bovinos. In: CONGRESSO DE INICIAÇÃO CIENTÍFICA, 26.; ENCONTRO DE PÓS-GRADUAÇÃO UFPEL, 19.; SEMANA INTEGRADA DE ENSINO, PESQUISA E EXTENSÃO, 3., 2017, Pelotas. Anais... Pelotas: UFPel, 2017.| Biblioteca(s): Embrapa Clima Temperado. |
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| 5. | | RINCON, J. A. A.; REMIÃO, M. H.; PREADIEÉ, J.; PEGORARO, L. M. C.; CORRÊA, M. N.; SCHNEIDER, A. Relação da lipoproteina de alta densidade durante a maturação oocitaria com o desenvolvimento embrionário inicial em bovinos. In: CONGRESSO DE INICIAÇÃO CIENTÍFICA, 26.; ENCONTRO DE PÓS-GRADUAÇÃO UFPEL, 19.; SEMANA INTEGRADA DE ENSINO, PESQUISA E EXTENSÃO, 3., 2017, Pelotas. Anais... Pelotas: UFPel, 2017.| Biblioteca(s): Embrapa Clima Temperado. |
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| 6. | | ACOSTA, D. A.; RINCÓN, J. A. A.; SILVEIRA, P. A. S.; PRADIEÉ, J.; CORREA, M. N.; PEGORARO, L. M. C.; SCHNEIDER, A. Effects of somatotropin injection on gene expression of follicular cells from the dominant follicle in cows. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 30., 2016, Foz do Iguaçu. Anais... Publicado na revista Animal Reproduction, v. 13, n. 3, p. 531, Jul./Sept. 2016.| Biblioteca(s): Embrapa Clima Temperado. |
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| 7. | | RINCÓN, J. A. A.; CAMPOS, F. T. DE; ACOSTA, D. A.; SILVEIRA, P. A. S.; GASPERIN, B. G.; CORREA, M. N.; PEGORARO, L. M. C.; SCHNEIDER, A. The effect of lipopolysaccharides on the expression of genes involved in steroidogenesis and inflammatory response in bovine granulosa cells. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 30., 2016, Foz do Iguaçu. Anais... Publicado na revista Animal Reproduction, v. 13, n. 3, p. 529, Jul./Sept. 2016.| Biblioteca(s): Embrapa Clima Temperado. |
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| 8. | | RINCÓN, J. A. A.; PREDIEÉ, J.; REMIÃO, M. H.; COLLARES, T. V.; MION, B.; GASPERIN, B. G.; ROVANI, M, T.; CORRÊA, M, N.; PEGORARO, L. M. C. Effect of high-density lipoprotein on oocyte maturation and bovine embryo development in vitro Reproduction in Domestic Animals, v.54, p. 445?455, 2019.| Biblioteca(s): Embrapa Clima Temperado. |
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| 9. | | RINCÓN, J. A. A.; GARCIA, D. N.; PRIETSCH, L.; VALLE, S. C.; HELBIG, E.; BARROS, C. C.; PEGORARO, L. M. C.; SCHNEIDER, A. Effects of a high-fat and energy diet on ovarian gene expression in young and aged female mice. Animal Reproduction., v.12, n.3, p. 584, Jul./Sept. 2015. Publicado: Proceedings of the 29th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Gramado, RS, Brazil, August 20th to 23rd, 2015, and 31st Meeting of the European Embryo Transfer Association (AETE); Ghent, Belgium,...| Biblioteca(s): Embrapa Clima Temperado. |
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| 10. | | RINCÓN, J. A. A.; MION, B.; ACOSTA, D. A. V.; GASPERIN, B. G.; ROVANI, M. T.; PEGORARO, L. M. C.; CORRÊA, M. N.; SCHNEIDER, A. Effect of recombinant bovine somatotropin (rbST) treatment on follicular population and development in non-lactating dairy cows. Animal Reproduction, v. 16, n. 4. p. 1-9, 2019.| Biblioteca(s): Embrapa Clima Temperado. |
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| 11. | | SILVA, J. F. da; RINCÓN, J. A. A; CAMPOS, F. T. de; ACOSTA, D. A. V.; PEGORARO, L. M. C.; SCHNEIDER, A. Atividade da paraoxonase-1 na fração hdl no soro e no fluido folicular de vacas jersey desafiadas com lipopolisacarideos. In: CONGRESSO DE INICIAÇÃO CIENTÍFICA, 25.; SEMANA INTEGRADA DE ENSINO, PESQUISA E EXTENSÃO, 2., 2016, Pelotas. Anais... Pelotas: UFPel, 2016.| Biblioteca(s): Embrapa Clima Temperado. |
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| 12. | | PRADIEÉ, J.; RINCÓN, J. A. A.; FRATA, M. M.; MION, B.; GINDRI, P. C.; ROVANI, M. T.; DIAS, J. dos S.; SCHNEIDER, A.; PEGORARO, L. M. C. Does lipopolysaccharide affect the rate of cleavage and embryonic development in vitro?. Reproduction in Domestic Animals, v. 14, n. 3, p. 851, jul/sept, 2017. Apresentado: Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo
Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.| Biblioteca(s): Embrapa Clima Temperado. |
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| 13. | | SILVA, J. F. da; VIEGAS, D. P.; MADEIRA, E. M.; CAMPOS, F.; LAGOS, K.; SILVA, E. F.; RINCÓN, J. A. A.; PEGORARO, L. M. C. Desenvolvimento de embriões ovinos produzidos in vitro com semen fresco e congelado usando o método de seleção espermática mini percoll. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA E PÓS-GRADUAÇÃO DA EMBRAPA CLIMA TEMPERADO, 5., 2014, Pelotas. Qual o papel da ciência na agricultura familiar?: anais. Pelotas: Embrapa Clima Temperado, 2015. 120 p.| Biblioteca(s): Embrapa Clima Temperado. |
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| 14. | | SILVA, J. F. da; RINCÓN, J. A. A.; SCHNEIDER, A.; CAMPOS, F. T. de; MADEIRA, E. M.; PEGORARO, L. M. C. Efeito da pon 1 durante a maturação oocitária sobre a expressão de genes relacionados ao estresse oxidativo de embriões bovinos. In: CONGRESSO DE INICIAÇÃO CIENTÍFICA, 24.; ENCONTRO DE PÓS-GRADUAÇÃO, 17., 2015, Pelotas. [Anais.]. Pelotas: UFPel, 2015.| Biblioteca(s): Embrapa Clima Temperado. |
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| 15. | | RINCÓN, J. A. A.; CAMPOS, F. T. de; ACOSTA, D. A. V.; SILVEIRA, P. A.; PEGORARO, L. M. C.; SCHNEIDER, A. Efeito do LPS sobre a expressão gênica em células da granulosa do folículo dominante de bovinos. In: CONGRESSO DE INICIAÇÃO CIENTÍFICA, 24.; ENCONTRO DE PÓS-GRADUAÇÃO, 17., 2015, Pelotas. [Anais.]. Pelotas: UFPel, 2015.| Biblioteca(s): Embrapa Clima Temperado. |
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| 16. | | HAX, L. T.; RINCÓN, J. A. A.; SILVA, J. F. da; PRADIEÉ, J.; PEGORARO, L. M. C.; SCHNEIDER, A.; CORREA, M. N. Effect of butafosfan in expression of genes associated oocyte quality. Reproduction in Domestic Animals, v. 14, n. 3, p. 752, jul/sept, 2017. Apresentado: Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo
Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.| Biblioteca(s): Embrapa Clima Temperado. |
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| 17. | | PREDIEÉ, J.; CAMPOS, F. T. de; RINCON, J. A. A.; COLLARES, L.; GOULARTE, K.; SILVEIRA, P. A. S.; PEGORARO, L. M. C.; SCHNEIDER, A. Paraoxonase 1 (PON1) activity in serum, follicular fluid and seminal plasma of sheep. Reproduction in Domestic Animals, mai, p. 1-3, 2017.| Biblioteca(s): Embrapa Clima Temperado. |
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| 18. | | COLLARES, L. F.; PRADIEÉ, J.; BORGES, M. A.; MION, B.; GINDRI, P.; FADRIQUE, J.; RINCÓN, J. A. A.; PEGORARO, L. M. C.; SCHEIDER, A.; CORRÊA, M. N. Supplementation of in vitro maturation medium with serum from dairy cows at early and late lactation in the in vitro production of bovine embryos. Medicina Veterinária, v.12, n. 4 , P. 311-315, 2018.| Biblioteca(s): Embrapa Clima Temperado. |
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| 19. | | MADEIRA, E.; MION, B.; SILVA, J. F. da; TORRES, F. C.; RINCON, J. A. A.; VEIRA, A. D.; PEGORARO, L. M. C.; LUCIA JUNIOR, T. Vitrificação de embriões bovinos (PIV) com etileno glicol monometil éter (EGMME): um novo crioprotetor. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA E PÓS-GRADUAÇÃO DA EMBRAPA CLIMA TEMPERADO, 5., 2014, Pelotas. Qual o papel da ciência na agricultura familiar?: anais. Pelotas: Embrapa Clima Temperado, 2015. 120 p.| Biblioteca(s): Embrapa Clima Temperado. |
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| 20. | | RINCON, J. A. A.; PRADIEÉ, J.; FEIJÓ, J. de O.; REMIÃO, M. H.; BORGES, M. A.; LPES, I. A. R.; CORREA, M. N.; PEGORARO, L. M. C.; SCHNEIDER, A. Effect of high density lipoprotein during in vitro oocyte maturation on initial embryo development in bovine. Reproduction in Domestic Animals, v. 14, n. 3, p. 749, jul/sept, 2017. Publicado: Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo
Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.| Biblioteca(s): Embrapa Clima Temperado. |
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| Registros recuperados : 27 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Clima Temperado; Embrapa Rondônia. |
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Data corrente: |
08/02/2017 |
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Data da última atualização: |
10/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
CAMPOS, F. T. de; RINCON, J. A. A.; ACOSTA, D. A. V.; SILVEIRA, P. A. S.; PRADIEÉ, J.; CORRÊA, M. N.; GASPERIN, B. G.; PFEIFER, L. F. M.; BARROS, C. C.; PEGORARO, L. M. C.; SCHNEIDER, A. |
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Afiliação: |
Felipe Terres de Campos, UFPel; Joao Aveiro Alvarado Rincon, UFPel; Diego Andres Velasco Acosta, CORPOICA; Pedro Augusto Silva Silveira, UFPel; Jorgea Pradieé, Embrapa Clima Temperado; Marcio Nunes Corrêa, UFPel; Bernardo Garziera Gasperin, UFPel; LUIZ FRANCISCO MACHADO PFEIFER, CPAF-Rondonia; Carlos Castilho Barros, UFPel; LIGIA MARGARETH CANTARELLI PEGORARO, CPACT; Augusto Schneider, UFPel. |
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Título: |
The acute effect of intravenous lipopolysaccharide injection on serumand intrafollicular HDL components and gene expression in granulosacells of the bovine dominant follicle. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Theriogenology, v. 89, p. 244 - 249, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to evaluate the effect of an acute systemic inflammatory response induced by lipopolysaccharide (LPS) in the serum and follicular fluid (FF) high-density lipoprotein (HDL) components, hormone concentrations and granulosa cell gene expression. For this purpose, twenty non-lactating Jersey dairy cows were submitted to a progesterone (P4) ? estradiol (E2) based synchronization protocol. Cows received a single i.v. dose of LPS (2.5 μg/kg of body weight) or saline solution (CTL Group) 2 h after P4 insert removal. Blood, granulosa cells and FF samples were collected six hours after LPS injection. Five hours after LPS injection rectal temperature was increased in LPS (P < 0.0001, 40.4 ± 0.1 °C) compared to the CTL cows (38.8 ± 0.1 °C). Serum PON1 activity was reduced by LPS injection (130.2 ± 5.1 vs. 99.6 ± 3.3 U/mL; P < 0.001), as well as HDL-cholesterol concentrations (70.3 ± 5.3 vs. 50.1 ± 6.2 mg/dL; P < 0.05). The FF E2 and P4 concentrations were not different between groups (P > 0.05). The PON1 activity in the FF was also decreased by LPS injection (P = 0.01). In comparison to CTL group, cows injected with LPS had a ten fold reduction in STAR, TLR4 and TNF mRNA expression (P < 0.05). In conclusion, an intravenous LPS challenge in cows induced an acute systemic inflammatory response reducing HDL and its components in serum but not in the FF. Only PON1 activity serum reduction was reflected in the FF in the short term. Additionally, steroidogenic and inflammatory genes had reduced expression in the granulosa cells. MenosThe aim of this study was to evaluate the effect of an acute systemic inflammatory response induced by lipopolysaccharide (LPS) in the serum and follicular fluid (FF) high-density lipoprotein (HDL) components, hormone concentrations and granulosa cell gene expression. For this purpose, twenty non-lactating Jersey dairy cows were submitted to a progesterone (P4) ? estradiol (E2) based synchronization protocol. Cows received a single i.v. dose of LPS (2.5 μg/kg of body weight) or saline solution (CTL Group) 2 h after P4 insert removal. Blood, granulosa cells and FF samples were collected six hours after LPS injection. Five hours after LPS injection rectal temperature was increased in LPS (P < 0.0001, 40.4 ± 0.1 °C) compared to the CTL cows (38.8 ± 0.1 °C). Serum PON1 activity was reduced by LPS injection (130.2 ± 5.1 vs. 99.6 ± 3.3 U/mL; P < 0.001), as well as HDL-cholesterol concentrations (70.3 ± 5.3 vs. 50.1 ± 6.2 mg/dL; P < 0.05). The FF E2 and P4 concentrations were not different between groups (P > 0.05). The PON1 activity in the FF was also decreased by LPS injection (P = 0.01). In comparison to CTL group, cows injected with LPS had a ten fold reduction in STAR, TLR4 and TNF mRNA expression (P < 0.05). In conclusion, an intravenous LPS challenge in cows induced an acute systemic inflammatory response reducing HDL and its components in serum but not in the FF. Only PON1 activity serum reduction was reflected in the FF in the short term. Additionally, steroidogenic a... Mostrar Tudo |
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Palavras-Chave: |
Esteroidogênese; PON1. |
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Thesagro: |
Colesterol; Inflamação. |
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Thesaurus NAL: |
cholesterol; inflammation; steroidogenesis. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227597/1/cpafro-17770.pdf
|
|
Marc: |
LEADER 02572naa a2200325 a 4500
001 2063230
005 2021-11-10
008 2017 bl uuuu u00u1 u #d
100 1 $aCAMPOS, F. T. de
245 $aThe acute effect of intravenous lipopolysaccharide injection on serumand intrafollicular HDL components and gene expression in granulosacells of the bovine dominant follicle.$h[electronic resource]
260 $c2017
520 $aThe aim of this study was to evaluate the effect of an acute systemic inflammatory response induced by lipopolysaccharide (LPS) in the serum and follicular fluid (FF) high-density lipoprotein (HDL) components, hormone concentrations and granulosa cell gene expression. For this purpose, twenty non-lactating Jersey dairy cows were submitted to a progesterone (P4) ? estradiol (E2) based synchronization protocol. Cows received a single i.v. dose of LPS (2.5 μg/kg of body weight) or saline solution (CTL Group) 2 h after P4 insert removal. Blood, granulosa cells and FF samples were collected six hours after LPS injection. Five hours after LPS injection rectal temperature was increased in LPS (P < 0.0001, 40.4 ± 0.1 °C) compared to the CTL cows (38.8 ± 0.1 °C). Serum PON1 activity was reduced by LPS injection (130.2 ± 5.1 vs. 99.6 ± 3.3 U/mL; P < 0.001), as well as HDL-cholesterol concentrations (70.3 ± 5.3 vs. 50.1 ± 6.2 mg/dL; P < 0.05). The FF E2 and P4 concentrations were not different between groups (P > 0.05). The PON1 activity in the FF was also decreased by LPS injection (P = 0.01). In comparison to CTL group, cows injected with LPS had a ten fold reduction in STAR, TLR4 and TNF mRNA expression (P < 0.05). In conclusion, an intravenous LPS challenge in cows induced an acute systemic inflammatory response reducing HDL and its components in serum but not in the FF. Only PON1 activity serum reduction was reflected in the FF in the short term. Additionally, steroidogenic and inflammatory genes had reduced expression in the granulosa cells.
650 $acholesterol
650 $ainflammation
650 $asteroidogenesis
650 $aColesterol
650 $aInflamação
653 $aEsteroidogênese
653 $aPON1
700 1 $aRINCON, J. A. A.
700 1 $aACOSTA, D. A. V.
700 1 $aSILVEIRA, P. A. S.
700 1 $aPRADIEÉ, J.
700 1 $aCORRÊA, M. N.
700 1 $aGASPERIN, B. G.
700 1 $aPFEIFER, L. F. M.
700 1 $aBARROS, C. C.
700 1 $aPEGORARO, L. M. C.
700 1 $aSCHNEIDER, A.
773 $tTheriogenology$gv. 89, p. 244 - 249, 2017.
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Embrapa Rondônia (CPAF-RO) |
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